This study confirms that the toxicity order of oxidants (sodium hypochlorite, hydrogen peroxide) to D.magna and Pseudorasbora parva is sodium hypochlorite > hydrogen peroxide. It also reveals that the suitable pH range for D. magna and Pseudorasbora parva is 5.4-10.2 and 4.2-10.8, respectively. Both species are highly tolerant to the turbidity caused by silicon dioxide and calcium fluoride (＞18000 NTU). The proposed toxicity identification evaluation (TIE) procedure was effectively used to confirm the toxicity by oxidants, turbidity, highly acidic and alkaline pollutants in high-tech industry effluents. Toxic and artificial industry effluents prepared by adding oxidants (sodium hypochlorite, hydrogen peroxide), turbidity (silicon dioxide, calcium fluoride), acidic and alkaline pollutants (hydrochloric acid, sodium hydroxide) were subjected to relevant physico-chemical treatments first. The toxicants were correctly identified and confirmed thereafter. The presence of hydrochloric acid and sodium hydroxide in water was effectively removed by simple aeration without adding any agent, representing a green and environmentally friendly treatment. Integration of toxicity identification evaluation procedures from the last three year achievements can efficiently identify the toxicity cause in Hsinchu Science Park high-tech industry plant water sample is by copper ions in HSP1 sample and copper/silver ions in HSP2, demonstrating its potential use for toxicity identification and evaluation in water body. Our preliminary result demonstrates that TIE procedure complemented to toxicity effect directed analysis method and integrated with Zebra fish embryonic chronic toxicity testing method developed in 2013 can effectively identify and evaluate potential toxicants in water body.

Biological effect、Toxicity identification evaluation、Effect-directed analysis、High-tech industry、Effluent